Our software package for smFRET and example smFRET data are available for download on GitHub.
Our code treats single-molecule time-binned FRET trajectories as hidden Markov processes, allowing one to determine the most likely FRET-value distributions of states and their interconversion rates based on probability alone. Source code available upon request.
HaMMy is available for download on GitHub.
Scale drawings of parts for Prism-type TIRF microscopy
Drawing of a brass objective heater
Drawing of prism extender
Drawing of prism holder
Drawing of prism holder from side
Drawing of prism holder with new spies
Drawing of prism slide holder
Drawing of stage
Drawing of stage with holes
PEG Protocol and Movie
PEG protocol as of September 2005
From Supplementary Information found in Rahul Roy, Sungchul Hohng, Taekjip Ha. “A practical guide to single-molecule FRET” Nature Methods (2008)
Email admins to request access to Ha and Myong Lab CPLS listserv.