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Our software package and example data for smFRET data acquisition and analysis are available for download at the Center for the Physics of Living Cells.
Our code treats single-molecule time-binned FRET trajectories as hidden Markov processes, allowing one to determine the most likely FRET-value distributions of states and their interconversion rates based on probability alone. Source code available upon request.
Scale drawings of parts for Prism-type TIRF microscopy
Drawing of a brass objective heater
Drawing of prism extender
Drawing of prism holder
Drawing of prism holder from side
Drawing of prism holder with new spies
Drawing of prism slide holder
Drawing of stage
Drawing of stage with holes